Development and Characterization of Guinea Pig Anti-Insulin Polyclonal Antibody.

Development and characterization of guinea pig anti-insulin polyclonal antibody against a target-specific insulin antigen. In India, an insulin immunogenicity kit for detecting insulin antibodies (neutralizing Nab) is an unmet medical need for diabetic patient's routine diagnosis. Type 1 diabetics rely on insulin injections daily basis for survival; if the body develops anti-insulin antibodies and neutralizes the exogenous recombinant insulin, glucose control is lost, and the patient eventually dies. Antibodies are excellent diagnostic reagents due to the specificity and sensitivity they provide in recognizing specific and unique target antigens. The paper describes the use of insulin as a target antigen and the development of target (insulin) specific antibodies in guinea pigs for use as a positive control for immunogenicity kit validation. Anti-insulin polyclonal antibody was raised against insulin in the Dunkin Hartley guinea pigs host. Anti-insulin antibody titer of all bleeds from four animals was tested using an indirect ELISA assay format. All four animals responded to the target-specific antigen but only one animal (#4) responded with a high-affinity antibody titer. The hyperimmune sera were purified using a protein A column. The purified anti-insulin antibody was characterized through SDS Page and western blot. The specificity, reactivity, and antibody binding efficiency were confirmed through immunoassays. Guinea pig anti-insulin polyclonal antibody developed in this study showed good specificity, reactivity, and efficiency in the immunoassays. This paper describes the development and characterization of anti-insulin antibodies for use as a control in developing a user-friendly insulin immunogenicity kit.

Biosynthesis of MgO nanoparticles using mushroom extract: effect on peanut (Arachis hypogaea L.) seed germination.

Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), and thermal gravimetric analysis (TGA) for average crystalline size, particle size, morphology, elemental analysis, and weight loss of the materials, respectively. This study reports the application of gardened sizes of (20, 18.5, 18, 16.5, and 15 nm) biosynthesized MgO NPs on seed germination. The smaller size (15 nm) MgO NPs have been enhanced the seed germination and growth parameters as compared with remaining sizes of MgO NPs and control. The magnesium oxide NPs penetrates into peanut seeds and affecting on seed germination and growth rate mechanism. In addition, this germination found to be high in seeds than germination on selected soil plot MgO NPs (0.5 mg/L stable concentrations) compared to different size of MgO NPs and control. Physicochemical methods indicated that the MgO NPs are able to penetrate into the seed coat and support water uptake inside of seeds. Probably, this positive effect may cause for the uptake of MgO NPs by the plants, as indicated in the UV and SEM analyses. As the smaller size (15 nm) of MgO NPs particles stimulates the development of seedling and growth enhancement of peanut, it clearly indicates that the current study is helpful in growing of peanuts in large-scale agricultural production.

Analysis of Reproductive Traits of Broiler Rabbits Reared in Sub-temperate Climate of Kodai Hills, Tamil Nadu, India.

AIM: The present study was carried out at Institute Rabbit Farm of Southern Regional Research Centre, Mannavanur, Kodaikanal, Tamil Nadu, India having sub-temperate climate with winter temperature during night hours going below 0°C with an objective of finding the influence of different factors such as breed, year, season and parity on different reproductive traits of broiler rabbits in order to come out with the best strategies for improving the productivity. MATERIALS AND METHODS: A total of 1793 records (946 White Giant and 847 Soviet Chinchilla) for weight at mating (WM), weight at kindling (WK), gestation length (GL), litter size at birth (LSB) and litter size at weaning (LSW), litter weight at birth (LWB), and litter weight at weaning (LWW) were collected in the period between 2000 and 2009 and the data was analyzed using general linear model option of SAS 9.2. RESULTS: The overall mean GL, WM, WK, LSB, LSW, LWB, and LWW were 31.68±0.04 days, 3.65±0.01 kg, 3.84±0.01 kg, 6.91±0.08, 5.49±0.09, 387.62±4.07 g, and 4.66±0.07 kg, respectively. The breed has significantly influenced GL, WK, LSW, LWB, and LWW. The LSB, LSW, LWB, and LWW were 7.05±0.11, 5.76±0.13, 399.55±5.88 g, and 4.87±0.10 kg, respectively in White Giant and corresponding values for Soviet Chinchilla were 6.78±0.11, 5.22±0.12, 375.91±5.64 g, and 4.46±0.09 kg, respectively. The year of kindling had significantly affected all the reproductive traits under study and is varying over different years. The parity significantly influenced the WM, WK, and LWW. The LWW increased from first (4.16±0.21 kg) to second parity (4.86±0.19 kg) and remained in the same range from third parity onward. WM was significantly higher in spring season (3.72±0.02) than the animals in rainy (3.59±0.02) and winter season (3.65±0.02). Better reproductive performance in terms of higher LSB, LSW, LWB, and LWW as observed in the present study might be due to conducive environmental conditions prevailing in the region. CONCLUSION: The significant effects of the non-genetic factors like year of kindling on all reproductive traits, season, and parity on some of the traits in rabbit breeds are indications that any future production enhancement strategy must take into consideration the environment by providing additional care, feed supplementation and better shelter management to the rabbits, so that the full genetic potential can be realized.

Emission analysis of RE3+ (RE = Sm, Dy):B2O3-TeO2-Li2O-AlF3 glasses.

This article reports on the optical properties of 0.5% mol of Sm(3+), Dy(3+) ion-doped B2O3-TeO2-Li2O-AlF3 (LiAlFBT) glasses. The glass samples were characterized by optical absorption and emission spectra. Judd-Ofelt theory was applied to analyze the optical absorption spectra and calculate the intensity parameters and radiative properties of the emission transitions. The emission spectra of Sm(3+) and Dy(3+):LiAlFBT glasses showed a bright reddish-orange emission at 598 nm ((4)G5/2 → (6)H7/2) and an intense yellow emission at 574 nm ((4)F9/2 → (6)H13/2), respectively. Full width at half maximum (FWHM), stimulated emission cross section, gain bandwidth and optical gain values were also calculated to extend the applications of the Sm(3+) and Dy(3+):LiAlFBT glasses.

Crystal growth and characterization of γ-glycine grown from potassium fluoride for photonic applications.

Single crystals of γ-glycine, an organic nonlinear optical material have been synthesized in the presence of potassium fluoride (KF) by slow evaporation technique at ambient temperature. The size of the grown crystal is up to the dimension of 12 mm×10 mm×8 mm. The γ-phase was confirmed by single crystal X-ray diffraction, powder XRD and the FTIR analysis. Optical absorption spectrum reveals that the grown crystal has good optical transparency in the entire visible region with an energy band gap of 5.09 eV, which is an essential requirement for a nonlinear optical crystal. Thermal stability of the grown γ-glycine crystal was determined using the thermo gravimetric and differential thermal analyses. The NLO activity of γ-glycine was confirmed by the Kurtz powder technique using Nd:YAG laser and the grown crystal exhibits high relative conversion efficiency when compared to potassium dihydrogen phosphate (KDP).

Seasonal variations in composting process of dead poultry birds.

Composting of dead birds with caged layer manure (CLM) and farm yard manure (FYM) was carried out to study the feasibility of composting as an alternative for disposal in Tamil Nadu State, India. The dead birds were sequentially layered with manure substrate and carbon source as per recipes formulated in mini-compost bins (4 x 4 x 4 feet). The temperature profile of both CLM and FYM group reached the peak by second week of composting and started declining steadily. The temperature profile was better during summer and monsoon and bins were able to maintain temperature above 60 degrees C (thermophilic) for 3-4 weeks. Season had no influence on attainment of peak temperature and it ranged between 51.8 and 70.4 degrees C. The persistency of thermophilic temperature (above 55 degrees C) was prolonged during summer (17.5-65 days) followed by monsoon (24-39 days) and winter (15.5-21.5 days). No putrefied or obnoxious odour or fly menace was observed during all the seasons of composting. The composting process took 107-127.5 days to finish during summer, 84.5-91 days in monsoon and 61.5-73.5 days in winter. The FYM was able to retain moisture higher (41-54% at the end of primary stage and 27.5-48.2% at the end of secondary stage) than CLM group (17.5-39.3% at primary stage and 20.4-33.5% in secondary stage). Weight reduction was more in FYM group (31.8-58.7%) than CLM group (19.3-48.6%). The volume reduction was uniform in all the seasons, it ranged between 39% and 59.3%.

Nonlinear control of bioreactors with input multiplicities--an experimental work.

The phrase input multiplicities means that an input variable with more than one value produces the same output value as if there were a single input-single output process. With input multiplicities, the value of the process gain changes as the manipulated variable changes, and beyond a certain input value, the sign of the gain also changes. A conventional PI controller for processes with input multiplicities may give unstable, less economical, or oscillatory responses. In the present work, control problems of a continuous bioreactor exhibiting two input multiplicities in the dilution rate on productivity were experimentally analyzed. A regulatory problem for the evaluation of controllers was taken up, i.e. a step change was made in the feed substrate concentration from 20 to 25 g/l at steady state conduction at lower (0.09386 h(-1)) and higher (0.2278 h(-1)) dilution rates for the same productivity of 2.9 g/l h. The nonlinear PI controller gave a more stable and fast response at both input dilution rates. The linear PI controller designed for a lower input dilution rate was stable, with some oscillations at the lower dilution rate, but the response was unstable at a higher dilution rate due to the input multiplicity behaviour of the process. Thus, nonlinear PI controller performance was found to be superior to that of the linear controller, and earlier reported theoretical results have been validated by the present experimental work.